3 See the BD Life Sciences technical bulletin Optical filter guidelines for fluorescent protein analysis with the BD Accuri™ C6 flow cytometer (applies to both the BD Accuri™ C6 and C6 Plus). BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. This portable and compact system holds the analytical power of a cytometer twice its size. Without the BD CSampler installation manual online for full details. NOTE: This update does not impact the BD Accuri™ C6 Plus. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> *Install the BD Accuri C6 Software. 4. In-Line Sheath Filter, Page 13: Figure 3-5. the BD Accuri C6 cytometer, saving time. Flow Cytometry Within Reach The analytical power and versatility of today’s laser-based flow cytometry systems Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus BD Accuri® C6 flow cytometer, certain items must be considered to ensure proper data acquisition. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Operate the instrument as described in this Instruction Manual. "WW�oÇ[�rs;$?�N8�)�#Nrb4'����ɻ����!�M�#���̹\W�_>u;t;?�x The volume In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. Sliding Luer Lock Fittings Off Of Pump Head, Page 25: Troubleshooting And Technical Support, Page 26: Figure 5-1. Start the Accuri CSampler software. BD Accuri C6 Plus software features an intuitive user interface. The following directions illustrate how to zoom in on this area. Software options … Windows Security Window. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of magnitude (a range of 0.34 to 80.9 pg). Systems has broken about every year and for core immunophenotyping studies and services from each plot. endobj • Calibrationshould be performedin the same tube type as the experimental sample. The BD CSampler adds minimal footprint to the BD Accuri C6, about three feet square for the pair, keeping the benchtop free for other uses. • For Information On Installing And Using The BD CSampler ™, See The BD Accuri CSampler … In-Line Sheath Filter, Page 23: Figure 4-4. This note discusses some of the basic concepts needed to perform small particle acquisition and analysis properly. The BD Accuri C6 Flow Cytometer System . Operate the instrument as described in this Instruction Manual. Technology Has Developed, And Reading Bd Accuri Analysis Manual Printable 2019 Books … BD Accuri C6 flow cytometer for the analysis of plant genome sizes. There are clearly two populations, and yet only one type of particle was run. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). There are two ways to make the identification: 1. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Take a leap in flow cytometry with the BD Accuri™ C6 Plus. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. x��XKo�H�#��� M��^EH �D�H���=x�!삙�1Q��V� ��m8ەz~]U]ݤ? ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' 1.1 About This Manual This manual provides information on cytometer … User's Guides and Manuals. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. 8. By default, BD Accuri C6 Software displays the area parameter (signified with a suffix of -A), but parameters can also be displayed in height, width (of primary threshold channel, only), or time. endobj We have got basic to find a instructions with no digging. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Turn on the computer and log in with your UCF info. For example, Figure 5 shows a 0.5-µm particle acquired on a BD Accuri C6 using an FSC trigger and threshold of 1,000. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. �(����-�G=I��e����AFk�����d7D����&V61{��P�IY��F�"����{$1�,�-�����k�!^-]�Q��o���1�j�5FU���5�5))�Zw�B���2��b��WT�q�Ĉ�a��{� WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. Peristaltic Pumps And Luer Locks, Page 24: Figure 4-6. Canada United States To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. !itwill#take# the#instrumentseveral#more#minutes#for#the#instrumentto#getto#the#greenElightready# … BD Accuri C6 with single tube loader protocol This protocol contains instructions for setting up an Accuri C6 with Tube Loader. <>>> 1 0 obj ���j��]o��P�Pwʄ�> ^��-3���˼�/�~Ϧ>4��bUTB G�%*��I��%Rh�"%�E �ѡ�*���g����π%��f�7�æ����.�J����Z:_,H�O<=/�#��gT��v_hǒ)pb�[�ڗ�R�5��mӶ�;�в���߷~�S����j�I��^�9d�8�G�{f�4"�Y�a4���Y��%�k���. 3. Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib detector(s). Of the BD Accuri C6 Flow Cytometer Instrument Manual Research Use Only. %���� 12. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide; Display of BD Accuri™ C6 Software-Generated FCS 3.0 Files Using FlowJo™ Version 8.8.7 or 9.4.11 for the Mac® Print version: Text Size: Related Files » C6 manual » BC aquatic uses ... Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). BD Accuri C6: BMSB J538. �&�Yl�H�$��9It��$�%�|X��̒��L���B��D��kt��/��#_� )a!�X܌��N�Xb��CF���D'�)A�K�ȃq��j���T����L�QO��< ��a�� Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). Comprehending as competently as deal even more than additional will find the money for each success. The Accuri C6 Flow Cytometer® simplifies cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' Proper Attachment Of Fluidics Lines, Page 15: Figure 3-8. REQUEST A DEMO ON A BD ACCURI C6 SYSTEM. Satchmo stanford shared facs facility (9 pages), Manual will be automatically added to "My Manuals", Laboratory Equipment BD CSampler Installation Manual, Laboratory Equipment BD LSR II User Manual, Laboratory Equipment BD FACSJAZZ User Manual, Laboratory Equipment BD FACSCanto User Manual, Laboratory Equipment BD FACSCanto Reference Manual, Laboratory Equipment BD FACSAria II Quick Reference Manual, Laboratory Equipment BD FACSort User Manual, Laboratory Equipment BD FACScan Instructions, Laboratory Equipment BD 644787 User Manual, Page 5: Figure 1-1. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. Open the Compensation Template from the desktop 2. Get Free Accuri C6 User Guide or third-party applications. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … All current service contracts will be honored. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for multiplex assays page, or contact our Technical Support team at technical@abcam.com. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Install, update, and run anti-virus protection software on a regular basis. Designed from the ground up to be compact and easy to use, the BD Accuri C6 … All current service contracts will be honored. The Bd Accuri C6 Flow Cytometer System, Page 6: Bd Accuri C6 Flow Cytometer Specifications, Page 9: Figure 3-1. We have got basic to find a instructions with no digging. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. Select Instrument > Calibrate Fluidics. Waste Line Quick Disconnect, Page 22: Figure 4-3. Default cytometer configuration for the Accuri C6 analyzer: For more information click the following links: C6 cytometer user manual. stream If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. 9. Within 5 minutes of completing the water run, place a calibration sample on the SIP. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. We have got basic to find a instructions with no digging. 新一代的Accuri使流式細胞術更加指日可待。 Taiwan North America. BD Accuri C6 Plus software features an intuitive user interface. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. Install BD CSampler software. Even new flow cytometry users find it so easy to learn and use that Scheduling. This is just one of the solutions for you to be successful. Nos. endobj BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Download: Bd Accuri Analysis Manual Printable 2019 Online Reading At BRAZILFILMFESTIVAL.INFO Free Download Books Bd Accuri Analysis Manual Printable 2019 We All Know That Reading Bd Accuri Analysis Manual Printable 2019 Is Beneficial, Because We Can Easily Get A Lot Of Information From Your Resources. 4 This filter transmits red laser light, which may damage the detector. The BD Accuri C6 Software installer is on the CD that came with the C6. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. To download BD ACCURI C6 MANUAL… Figure 6 shows how the FCS file data will look when initially opened in FlowJo. 7820095-01 Rev-0 51. Improper Shut Down Message, Page 18: Figure 4-1. To download BD ACCURI C6 MANUAL… To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Use another characteristic of the particle, such as fluorescence, to (In this example, the file is FCS Express Import Demo.c6.) Core Size/Flow Rate Combinations.....79 Figure C-1. 1. • For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User Guide. A Guide to Absolute Counting Using the BD Accuri® C6 Flow Cytometer BD Biosciences August 2011 Page 3 5. User Tracking Installer Icon .....80 Figure C … Install, update, and run anti-virus protection software on a regular basis. Place the mat in position (Figure 10). Open the lid of the BD Accuri C6 and locate the holes used to mount the BD CSampler (Figure 4). 3 0 obj To perform compensation, 1. Please complete the following information to request a demonstration of the BD Accuri™ C6 flow cytometer. 2 0 obj <> As understood, achievement does not recommend that you have fantastic points. So the choice should be addressed by the kind of flow experiments you plan to do. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. DI water, Cat. BD Accuri C6 Software User Guide - Manual. NOTE: This update does not impact the BD Accuri™ C6 Plus. On the Accuri C6, all compensation is performed manually. BD Accuri® - Guide to the FCS Express™ BD Accuri CFlow® Importer Instruction Manual Subject: The FCS Express BD Accuri CFlow Importer, available in FCS Express version 4, allows you to automatically recreate any analysis scheme from BD Accuri CFlow® or BD Accuri CFlow Plus software in FCS Express. Plug in the power cord and turn on the C6. 10. Designed from the ground up to be compact and C6 software (Cflow Plus) software manual. User Tracking Installer Icon .....80 Figure C … It Also Includes Common Troubleshooting Help And Service Information. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Holes Figure 4 Figure 5 Figure 6 5. BD CSampler Software User Guide iv 7820099-01 Rev-2 TABLE OF FIGURES Figure 1-1. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. 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